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34321-M

Sigma-Aldrich

FITC, Isomer I

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Synonym(s):
FITC, Isomer I, Fluorescein Isothiocyanate, Isomer I
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
MDL number:

Assay

≥95% (TLC)

Quality Level

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

orange-red

solubility

DMSO: 5 mg/mL
DMF: soluble
aqueous buffer, pH ≥ 9.0: soluble

storage temp.

2-8°C

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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This Item
F4274F725046951
FITC, Isomer I

Sigma-Aldrich

34321-M

FITC, Isomer I

form

solid

form

powder

form

powder

form

powder

color

orange-red

color

-

color

-

color

-

solubility

DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble

solubility

acetone: soluble 10 mg/mL

solubility

acetone: 1 mg/mL

solubility

acetone: soluble 0.1 g/10 mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

General description

A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9). Fluorescence EMmax: 520 ± 3 nm (pH 9).
Popular derivative of fluorescein. Can be used to label antibodies and other proteins.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Cherry, N.B., et al. 1965. Stain Technol. 44, 179.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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