344289
Freund′s Complete Adjuvant, Modified, Mycobacterium butyricum
This Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum is validated for use in Immunization.
Synonym(s):
Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 set | Check Cart for Availability | $318.00 |
About This Item
biological source
(Heat killed Mycobacterium tuberculosis)
Quality Level
description
RTECS - LS6860000
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunization)
color
clear to slightly cloudy amber
shipped in
ambient
storage temp.
2-8°C
General description
1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.
Contains 10 vials per set, each containing 10 ml of Freund′s complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.
Application
Immunization
Physical form
Yellow, oily, bacterial suspension.
Other Notes
Composition
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| biological source (Heat killed Mycobacterium tuberculosis) | biological source - | biological source (Synthetic chemical) | biological source - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 10-30°C | storage temp. 2-8°C |
| shipped in ambient | shipped in - | shipped in ambient | shipped in ambient |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| form liquid | form cell suspension | form liquid (oily) | form powder |
| Quality Level 100 | Quality Level 300 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Asp. Tox. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 344289-1SET | 04055977214697 |