Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

345763

Sigma-Aldrich

GDF-9, Mouse, Recombinant

GDF-9, Mouse, Recombinant, consists of amino acids 307-477 and is expressed in Chinese Hamster Ovary cells. An oocyte secreted paracrine factor that is necessary for mammalian folliculogenesis.

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203

recombinant

expressed in CHO cells

Quality Level

assay

≥95% (SDS-PAGE)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

General description

Recombinant, mouse growth differentiation factor 9 (GDF-9) consisting of amino acids 307-477 and expressed in Chinese Hamster Ovary cells. GDF-9 is an oocyte secreted paracrine factor, necessary for mammalian ovarian folliculogenesis. Mature mouse GDF-9 is 90% identical to the amino acid sequence of mature human GDF-9. GDF-9 as a paracrine factor is useful in granulosa cell proliferation and differentiation regulation.

Biochem/physiol Actions

Effective concentration = 100 ng/ml as measured by induction of Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered HCl, BSA.

Reconstitution

Reconstitute to 100 µg/ml in 4 mM HCl containing 0.1% bovine serum albumin.

Other Notes

Mottershead, D.G., et al. 2008. Mol. Cell. Endocrinol.283, 58.
Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.
Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.
Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.
Roh, J.S., et al. 2003. Endocrinology144, 172.
Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.
Dong, J., et al. 1996. Nature383, 531.
McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.
McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service