346104

Plk2, GST- Fusion, Human, Recombinant, S. frugiperda

• Synonym(s): Polo-like kinase 2, Human, Recombinant
• UNSPSC Code: 12352202

Properties

• assay: ≥70% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.

Physical form

In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween^®-20 detergent, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Eckerdt, F., et al. 2005. Oncogene24, 267.
Warnke, S., et al. 2004.Curr Biol.14, 1200.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1