354406
Glutaredoxin-S2, E. coli
Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase.
Synonym(s):
Grx1, grxA
About This Item
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Quality Level
form
lyophilized
specific activity
≥175 μmole/min-mg (NADPH oxidized)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
protein purification: suitable
NCBI accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Escherichia coli ... grxB(946926)
General description
Glutaredoxins (GRXs) are small thiol belonging to the Thioredoxin (TRX) superfamily. Different isoforms with varied functions have been identified in E.coli namely: glutaredoxin 1, glutaredoxin 2, and glutaredoxin 3. [1] Native glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
Application
- to catalyze the reduction of S-glutathionylated cysteine residues, to facilitate the detection of protein S-glutathionylation in FFPE HLTF-/-CDX sections.[2]
- for reduction of the protein sulfhydryl groups that were modified by glutathione for subsequent labeling and purification of glutathionylated mitochondrial proteins.[3][4]
Biochem/physiol Actions
Warning
Unit Definition
Physical form
Reconstitution
Other Notes
Sun, C., et al. 1997. Protein Sci.6, 383.
Prinz, W.A., et al. 1997. J. Biol. Chem.272, 15661.
Holmgren, A. and Alund, F. 1995 Methods Enzymol.252, 283.
Hoog, J.O., et al. 1986. Gene43, 13.
Legal Information
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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