362300

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

• Synonym(s): N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli, Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F
• CAS Number: 83534-39-8
• EC Number: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥10 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• foreign activity: β-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.

Physical form

Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.

One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable