This recombinant enzyme is provided in an active form.
368044
Granzyme A, Human, Recombinant, E. coli
Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.
Synonym(s):
GzmA
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10 μG
$443.10
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10 μG
$443.10
About This Item
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recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥100 units/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
1 of 4
This Item | 407293 | 407654 | 407617 |
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form liquid | form liquid | form lyophilized | form lyophilized |
| specific activity ≥100 units/μg protein | specific activity ≥3.5 x 104 units/mL | specific activity - | specific activity - |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze | storage condition OK to freeze |
| shipped in wet ice | shipped in wet ice | shipped in ambient | shipped in ambient |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
General description
Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
Physical form
In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
Other Notes
Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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