374805

Sigma-Aldrich

Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, KLH
CAS Number:
MDL number:

storage conditions

+2C to +8C

Quality Level

assay

≥90% (size exclusion chromatography)

form

lyophilized solid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

blue to blue-gray

shipped in

ambient

storage temp.

2-8°C

General description

Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.

Packaging

1 g in Plastic ampoule
250 mg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from BES buffer, MgSO₄.

Reconstitution

Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).

Other Notes

Protein concentration: ≥60%

Copper: ≥0.09%

Copper to Protein ratio: ≥1.5 x 10-3
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

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