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Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ buffer, CAS 9013-72-3, is a large, multi-subunit, O2-carrying, metalloprotein that is used in the production of antibodies.

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Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer, KLH
CAS Number:
MDL number:

Quality Level

assay

≥90% (size exclusion chromatography)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

color

clear blue-gray

shipped in

wet ice

storage temp.

2-8°C

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374825H7017H8283
Quality Level

100

Quality Level

200

Quality Level

300

Quality Level

300

form

liquid

form

liquid

form

powder

form

PBS solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

do not freeze

storage condition

do not freeze

storage condition

-

storage condition

-

color

clear blue-gray

color

clear bluish

color

-

color

-

General description

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. It is isolated from a marine snail species called Megathura crenulate.

Biochem/physiol Actions

Keyhole limpet hemocyanin (KLH) exhibits immunostimulatory properties and stimulates both cell-mediated and humoral responses in animals and humans. It serves as a vaccine adjuvant and facilitates protein conjugation. In addition, KLH acts as a carrier protein to produce antibodies as well as haptens such as peptides and oligosaccharides. It is also used in the treatment of bladder cancer.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM MgSO₄, 5 mM BES buffer, 0.9% NaCl, pH 6.5. Sterile-filtered.

Preparation Note

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray
Oyelaran O and Gildersleeve JC, et al.
4th International Conference on Biomedical Engineering in Vietnam, 285-294 (2010)
Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it

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