374817

Sigma-Aldrich

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, KLH
CAS Number:
MDL number:

storage conditions

+2C to +8C

Quality Level

assay

>90% (size exclusion chromatography)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

clear blue-gray

shipped in

ambient

storage temp.

2-8°C

General description

Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.

Packaging

1 g in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.

Preparation Note

Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.

Other Notes

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 2

Certificate of Analysis

Certificate of Origin

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