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Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

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Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
CAS Number:
MDL number:

Quality Level

Assay

≥95% (size exclusion chromatography)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

impurities

≤25 EU/mg Endotoxin
≥1.5 x 10⁻³ copper to protein ratio
≥5 mg/mL Protein

color

clear bluish

shipped in

wet ice

storage temp.

2-8°C

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This Item
H7017H8283374813
form

liquid

form

powder

form

PBS solution

form

liquid

storage condition

do not freeze

storage condition

-

storage condition

-

storage condition

do not freeze

impurities

≤25 EU/mg Endotoxin, ≥1.5 x 10⁻³ copper to protein ratio, ≥5 mg/mL Protein

impurities

-

impurities

-

impurities

≤12 EU/mg Endotoxin, ≥1.5 x 10⁻³ copper to protein ratio, ≥4 mg/mL Protein

color

clear bluish

color

-

color

-

color

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. This high molecular weight glycoprotein is extracted from a marine snail species called Megathura crenulate.

Application

Hemocyanin, Keyhole Limpet, Megathura crenulata, high purity, endotoxin-free, sterile-liquid has been used as an antigen to test the polyreactivity of monoclonal antibody (mAb) using enzyme-linked immunosorbent assay(ELISA). It has also been used to immunize animals to study KLH-specific ex vivo recall responses and specific T-cell dependent antibody response (TDAR) in monkeys.

Biochem/physiol Actions

Keyhole limpet hemocyanin (KLH) stimulates both cell-mediated and humoral responses in animals and humans. It elicits immunostimulatory properties and also functions as a vaccine adjuvant. KLH serves as a carrier protein to produce antibodies and haptens like peptides and oligosaccharides. It is used to treat bladder cancer.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 83 mM sodium phosphate, 150 mM NaCl, pH 7.2.

Preparation Note

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Joshua T Gamse et al.
Toxicology and applied pharmacology, 409, 115285-115285 (2020-10-19)
The OX40 receptor plays a crucial co-stimulatory role in T effector cell survival, expansion, cytokine production, and cytotoxicity to tumor cells; therefore, OX40 agonists are being evaluated as anti-cancer immunotherapies, especially in combination with checkpoint inhibitors. To support clinical development
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it
Jenna J Guthmiller et al.
Immunity, 53(6), 1230-1244 (2020-10-24)
Polyreactivity is the ability of a single antibody to bind to multiple molecularly distinct antigens and is a common feature of antibodies induced upon pathogen exposure. However, little is known about the role of polyreactivity during anti-influenza virus antibody responses.

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