382155

Histone Deacetylase Substrate, Fluorogenic

• Synonym(s): Histone Deacetylase Substrate, Fluorogenic, Boc-Lys(Ac)-AMC, MAL, N-(4-Methyl-7-coumarinyl)-N ^α-(t-butoxycarbonyl)-N ^ω-acetyllysinamide
• Empirical Formula (Hill Notation): C₂₃H₃₁N₃O₆
• CAS Number: 233691-67-3
• Molecular Weight: 445.51
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: ethanol: 1 mg/mL; DMSO: 5 mg/mL
• fluorescence: λ_ex 330 nm; λ_em 395 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C23H31N3O6/c1-14-12-20(28)31-19-13-16(9-10-17(14)19)25-21(29)18(8-6-7-11-24-15(2)27)26-22(30)32-23(3,4)5/h9-10,12-13,18H,6-8,11H2,1-5H3,(H,24,27)(H,25,29)(H,26,30)/t18-/m0/s1
• InChI key: SUTRDULVNIPNLW-SFHVURJKSA-N

Description

General description

A fluorogenic substrate for histone deacetylase (HD).

A fluorogenic substrate for histone deacetylase (HD; excitation maximum: 330 nm; emission maximum: 395 nm).



Note: The histone deacetylase will cleave the Ac group and after the enzymatic reaction 2 peaks will be visible on HPLC. Before the reaction HPLC shows only one peak. The measure of second peak gives the degree of hydrolysis. It is important to use HPLC equipped with a fluorescence detector.

Warning

Toxicity: Standard Handling (A)

Other Notes

Hoffmann, K., et al. 1999. Nucleic Acids Res.27, 2057.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable