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Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda

Histone Deacetylase 8, His•Tag, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.

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Synonym(s):
HDAC8, Human, Recombinant

recombinant

expressed in insect cells (S. frugiperda)

Quality Level

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥10 units/μg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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This Item
382180171539524733
specific activity

≥10 units/μg protein

specific activity

≥50 units/μg protein

specific activity

≥270 nmol/min-mg protein

specific activity

≥0.25 U/μL

form

liquid

form

liquid

form

liquid

form

liquid

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in insect cells (S. frugiperda)

recombinant

-

recombinant

expressed in insect cells (S. frugiperda)

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.

Physical form

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Waltregny, D., et al. 2005. FASEB J.19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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