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391338

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HEPES

ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

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Synonym(s):
HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:

Quality Level

Assay

≥99% dry basis (titration)

form

crystalline powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 1 M

anion traces

chloride (Cl-): ≤0.01%
sulfate (SO42-): ≤0.05%

cation traces

Na: ≤0.1%
heavy metals: ≤1 ppm

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
54457H23830H7523
HEPES ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Millipore

391338

HEPES

-
HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

Premium Grade
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

Essential Grade
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

Premium Grade
form

crystalline powder

form

powder

form

crystalline

form

powder

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

color

white

color

-

color

-

color

-

solubility

water: 1 M

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

-

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.01%, sulfate (SO42-): ≤0.05%

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

anion traces

-

anion traces

chloride (Cl-): ≤0.05%

General description

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
Absorbance (1.0 M, H2O, 260 nm):≤0.05.

Absorbance (1.0 M, H2O, 280 nm): ≤0.04

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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