400052

Millipore

Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Synonym(s):
Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
Molecular Weight:
527.52
MDL number:
Pricing and availability is not currently available.

storage conditions

+2C to +8C

Quality Level

assay

≥85% (HPLC and TLC)

form

liquid

mfr. no.

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1

InChI key

GRRNUXAQVGOGFE-KPBUCVLVSA-N

General description

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
Sterile liquid formulation of Cat. No. 400050.

Packaging

20, 5, 50 ml in Alu drum

Application

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Biochem/physiol Actions

Potency: ≥1000 U/mg

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Physical form

50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.

Other Notes

Sugimoto, K., et al. 1994. Plant J.5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.34, 1915.
Cullen, D., et al. 1987. Gene57, 21.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Santerre, R.F., et al. 1984. Gene30, 147.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Risk Statement

26/27/28-37/38-41

Safety Statement

23-26-36/37/39-45

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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