400064

Sigma-Aldrich

Anti-Glucose Transporter-4 Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-Glut-4
Pricing and availability is not currently available.

storage conditions

-20C

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

mfr. no.

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

General description

Recognizes the ~40-43 kDa GLUT-4 protein.
Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.

Immunogen

a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
Mouse

Packaging

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application

ELISA (0.5-1 µg/ml)

Frozen Sections (2-20 µg/ml)

Immunoblotting (1-10 µg/ml; see comments)

Immunoprecipitation (1-10 µg/100 µg tissue membrane)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 0.1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Young Ju Lee et al.
Molecular medicine reports, 7(5), 1571-1578 (2013-04-03)
Alzheimer's disease (AD) is closely associated with significant defects in glucose metabolism. To investigate whether AD pathology induced by overexpression of human mutant presenilin 2 (PS2) protein induces changes in glucose metabolism, glucose‑related factors were analyzed in the brain of...
Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle.
Taylor, EB; An, D; Kramer, HF; Yu, H; Fujii, NL; Roeckl, KS; Bowles, N; Hirshman, MF; Xie et al.
The Journal of Biological Chemistry null

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.