Recognizes the ~40-43 kDa GLUT-4 protein.
Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
ELISA (0.5-1 µg/ml)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
Toxicity: Standard Handling (A)
In PBS, 0.1% BSA, pH 7.5.
Following initial thaw, aliquot and freeze (-20°C).
Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany