MilliporeSigma
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401459

Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

liquid, Calbiochem®

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

secondary antibodies

clone

monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.

Packaging

250 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application

ELISA (see comments)

Enzyme Immunoassay (≥1:5000)

Immunoelectrophoresis (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Other Notes

Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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