407255

IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant

• Synonym(s): Farnesyl Proteintransferase, FTase, IGFBP-7
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• solubility: soluble (Reconstitute to 100 µg/ml in sterile PBS.)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.

Biochem/physiol Actions

Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS.

Preparation Note

Reconstitute to 100 µg/ml in sterile PBS.

Other Notes

Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612.
Nagakubo, D. et al. 2003. J. Immunol.171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ.13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747.
Kato, M.V. 2000. Mol. Med.6, 126.
Hwa, V. et al. 1999. Endocr. Rev.20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981.
Kato, M.V. et al. 1996. Oncogene12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1