407256

IGF-II, CF, Mouse, Recombinant

• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, mouse insulin-like growth factor II (IGF-II) consisting of amino acids 25-91 and expressed in E. coli. Mouse IGF-II is synthesized as a 180 amino acid preproprecursor. Mature IGF-II is 91% and 97% amino acid identical to human and rat IGF-II, respectively.

Biochem/physiol Actions

ED₅₀ = 2-10 ng/ml as measured in a serum-free cell proliferation assay using MCF-7 human breast adenocarcinoma cells.

Physical form

Lyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS.

Other Notes

Ostrovsky, O., et al. 2009. Mol. Biol. Cell20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev.19, 111.
Belfiore, A., 2007. Curr. Pharm. Des.13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab.292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol.7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res.125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des.13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v.16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett.195,127.
Buchanan, C.M., et al. 2001. Biochem. J.360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci.57, 932.
Goya, L., et al. 1999. J. Biol. Chem.274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept.48, 77.
McCarthy, T.L., et al. 1992. Endocrinology130, 1303.
Zindy, F., et al. 1992. J. Hepatol.14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol.10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA85, 265.
Stempien, M.M., et al. 1986. DNA5, 357.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 4.1B - Flammable solid hazardous materials
• wgk_germany: WGK 2