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407617

Sigma-Aldrich

Interleukin-1β, Mouse, Recombinant, E. coli

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assay

≥97% (SDS-PAGE)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1.0 EU/μg Endotoxin (EU/μg IL-1β)

shipped in

ambient

Storage temp.

−70°C

General description

Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.
Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Interleukin-1 is a protein produced by stimulated macrophages and many other cell types. The following biological activities are attributed to IL-1: activation of T cells resulting in the production of IL-2 and expression of IL-2 receptors; initiation of Acute Phase Response by induction of hepatic protein synthesis; increased proteolysis of muscle proteins, amino acid mobilization, and caloric and oxygen utilization; induction of bone resorption; stimulation of fibroblast proliferation; induction of slow-wave sleep; fever production; induction of prostaglandin and leukotriene production; stimulation of thymocyte proliferation; induction of B cell proliferation and maturation and increased immunoglobulin synthesis; induction of collagenase and other proteinases in synovial fibroblasts and articular chrondrocytes, suggesting a role in pathological states such as arthritis; activation of neutrophils. 1 µg is equivalent to 1.1 x 106 WHO units.

Biochem/physiol Actions

ED₅₀= 5-10 pg/ml as measured in a cell proliferation assay

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Other Notes

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Weidmann, B., et al. 1994. Nature370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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