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410957

Sigma-Aldrich

3-Isobutyl-1-methylxanthine - CAS 28822-58-4 - Calbiochem

A cell-permeable, non-specific inhibitor of cAMP and cGMP phosphodiesterases (IC₅₀ = 2-50 µM).

Synonym(s):
3-Isobutyl-1-methylxanthine - CAS 28822-58-4 - Calbiochem, IBMX
Empirical Formula (Hill Notation):
C10H14N4O2
CAS Number:
Molecular Weight:
222.24
MDL number:

Quality Level

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

ethanol: 10 mg/mL
DMSO: 100 mM
methanol: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C10H14N4O2/c1-6(2)4-14-8-7(11-5-12-8)9(15)13(3)10(14)16/h5-6H,4H2,1-3H3,(H,11,12)

InChI key

APIXJSLKIYYUKG-UHFFFAOYSA-N

General description

A non-specific, cell-permeable inhibitor of cAMP and cGMP phosphodiesterases (IC50 = 2-50 µM). Also acts as an adenosine receptor antagonist. Reported to inhibit TNFα expression in adipocyte precursor cells.
A cell-permeable, non-specific inhibitor of cAMP and cGMP phosphodiesterases (IC50 = 2-50 µM). Also acts as an adenosine receptor antagonist. Reported to inhibit TNF-α expression in adipocyte precursor cells.

Packaging

1 g in Plastic ampoule
250 mg in Plastic ampoule

Biochem/physiol Actions

Product does not compete with ATP.
Cell permeable: yes
Primary Target
cAMP and cGMP phosphodiesterases
Reversible: no
Target IC50: 2-50 µM against cAMP and cGMP phosphodiesterases

Warning

Toxicity: Standard Handling (A)

Preparation Note

Heating may be required to achieve complete solubilization.

Reconstitution

Following reconstitution in ethanol or methanol, refrigerate (4°C). Following reconstitution in DMSO, aliquot and freeze (-20°C). Ethanol/methanol and DMSO stock solutions are stable for up to 6 months at 4°C or -20°C, respectively.

Other Notes

Hube, F., et al. 1999. Horm. Metab. Res.31, 359.
Morgan, A.J., et al. 1993. Biochem. Pharmacol.45, 2373.
Scamps, F., et al. 1993. Eur. J. Pharmacol. 244, 119.
Turner, N.C., et al. 1993. Br. J. Pharmacol. 108, 876.
Tamura, T., et al. 1991. J. Gen. Physiol. 98, 95.
Beavo, J.A., and Reifsnyder, D.H. 1990. Trends Pharmacol. Sci.11, 150.
Russell, T.R. 1979. Proc. Natl. Acad. Sci. USA76, 4451.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Quality

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