419700

Isopeptidase T, Rabbit

• Synonym(s): Ipase-T
• UNSPSC Code: 12352202

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity.

Biochem/physiol Actions

Fully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Dang, L.C., et al. 1998. Biochemistry 37, 1868.
Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
Stein, R.L., et al. 1995. Biochemistry 34, 12616.
Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable