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420108

Sigma-Aldrich

JNK Substrate, c-Jun 1-79

Synonym(s):

SAPK Substrate

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About This Item

UNSPSC Code:
12352200

assay

≥90% (SDS-PAGE)

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−70°C

General description

GST fusion protein of c-jun (1-79) activation domain, expressed in E. coli. Highly specific substrate form c-jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Not phosphorylated by MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity.
Recombinant c-Jun (1-79) activation domain fused to GST and expressed in E. coli. Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Is not phosphorylated by p42 MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity. Shown to undergo phosphorylation by JNK in the presence of 32P-ATP.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)
Product does not compete with ATP.
Reversible: no
Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Derijard, B., et al. 1994. Cell 76, 1025.
Kyriakis, J.M., et al. 1994. Nature369, 156.
Hibi, M., et al. 1993. Genes Dev.7, 2135.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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