437695

Sigma-Aldrich

Anti-Lipoic Acid Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-LA
Pricing and availability is not currently available.

storage conditions

= -70C

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity (predicted by homology)

all

mfr. no.

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

General description

Rabbit polyclonal antibody supplied as diluted serum. Recognizes native lipoic acid and lipoic acid covalently attached to proteins.
Recognizes native lipoic acid. Does not recognize lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products.
This Anti-Lipoic Acid Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Lipoic Acid.

Immunogen

lipoic acid conjugated to KLH

Packaging

100 μL in Plastic ampoule

Application

ELISA (1:500-1:1000)

Frozen Sections (1:50-1:500)

Immunoblotting (1:1000-1:10,000)

Paraffin Sections (1:50-1:500)

Warning

Toxicity: Standard Handling (A)

Physical form

serum diluted in buffer, 50% glycerol, pH 7.5.

Other Notes

Sasaki, M., et al. 2000. J. Autoimmun.15, 51.
Heitzer, T., et al. 2001. Free Radic. Biol. Med.31, 53.
Patrick. L. 2000. Altern. Med. Rev.5, 290.
Shi, S.S., et al. 1999. Antioxid. Redox Signal1, 123.
Humphries, K.M. and Szeda, L.I. 1998. Biochemistry37, 15835.
Does not bind lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products. Useful for assessing whether protein-bound lipoic acids have undergone certain forms of oxidative modification. The presence of protein-bound lipoic acid can be used as a marker for mitochondria. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Certificate of Analysis
Certificate of Origin
null
null
E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity.
Lacroix, et al.
Proceedings of the National Academy of Sciences of the USA, 113, 10998-11003 (2020)
Jorge Araya-Flores et al.
Plant physiology and biochemistry : PPB, 151, 264-270 (2020-04-04)
Lipoic acid (LA) and its reduced form (dihydrolipoic acid, DHLA) have unique antioxidant properties among such molecules. Moreover, after a process termed lipoylation, LA is an essential prosthetic group covalently-attached to several key multi-subunit enzymatic complexes involved in primary metabolism...
Nathan P Ward et al.
The Journal of experimental medicine, 217(6) (2020-03-21)
Human lung tumors exhibit robust and complex mitochondrial metabolism, likely precipitated by the highly oxygenated nature of pulmonary tissue. As ROS generation is a byproduct of this metabolism, reducing power in the form of nicotinamide adenine dinucleotide phosphate (NADPH) is...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.