437707

Lipase, Chromobacterium viscosum

• Synonym(s): Lipase, Chromobacterium viscosum
• CAS Number: 9001-62-1
• EC Number: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00011494
• UNSPSC Code: 12352202
• NACRES: NA.54
• Specific activity: ≥2500 units/mg dry wt.

Properties

• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N
• description: Merck USA index - 14, 5511
• form: solid
• specific activity: ≥2500 units/mg dry wt.
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: off-white
• pI: (1) 3.7, (2) 7.3
• shipped in: ambient
• storage temp.: −20°C
• Quality Level: 100

Description

General description

Native lipase from Chromobacterium viscosum. Catalyzes the hydrolysis of triglycerides to glycerol and fatty acids. Has a pI of 3.7 and 7.3.

Note: 1 KU = 1000 units.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Reconstitute in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA.

Other Notes

One unit is defined as the amount of enzyme that will release 1.0 µmol fatty acids from olive oil per min at 37°C, pH 8.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable