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437996

Sigma-Aldrich

5-Lipoxygenase, Human, Recombinant, S. fruigiperda

5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.

Synonym(s):
5-LO
Enzyme Commission number:

Quality Level

form

liquid

specific activity

~20 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.

Packaging

500 u in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.

Physical form

In 100 mM Tris containing 5 mM EGTA, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Brock, T.G., et al. 1997. J. Biol. Chem.272, 8276.
Pouliot, M., et al. 1996. Eur. J. Biochem.238, 250.
Zhang, Y.Y., et al. 1993. J. Biol. Chem.268, 2535.
Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA81, 689.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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