444236

Sigma-Aldrich

Anti-MMP-9 Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-Gelatinase B, Anti-Matrix Metalloproteinase 9

storage conditions

= -70C

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, marmoset

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

General description

This Anti-MMP-9 Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of MMP-9.
Recognizes all forms of MMP-9 under reducing and non-reducing conditions. Exhibits only slight cross-reactivity with MMP-2. Does not cross-react with MMP-1, MMP-3, or MMP-8.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes all forms of MMP-9 under reducing and non-reducing conditions.

Immunogen

human pro-MMP-9

Packaging

500 μL in Plastic ampoule

Application

ELISA (see comments)

Immunoblotting (1:500-1:1000)

Immunofluorescence (1:50-1:250)

Immunohistochemistry (1:40-1:150)

Immunoprecipitation (1:20-1:60)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Exhibits very slight reactivity with MMP-2. Does not cross-react with collagenases, MMP-1, MMP-3, or MMP-8. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Kolkenbrock, H., et al. 1996. Biol. Chem.377, 529.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

M Diessler et al.
Reproduction in domestic animals = Zuchthygiene, 52(1), 35-43 (2016-11-20)
The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the...
Nicolas T Kummer et al.
Journal of cellular biochemistry, 113(6), 1998-2008 (2012-01-19)
Arachidonate 5-lipoxygenase (ALOX5) expression and activity has been implicated in tumor pathogenesis, yet its role in papillary thyroid carcinoma (PTC) has not been characterized. ALOX5 protein and mRNA were upregulated in PTC compared to matched, normal thyroid tissue, and ALOX5...
Anna Michopoulou et al.
Matrix biology : journal of the International Society for Matrix Biology, 94, 1-17 (2020-07-06)
Re-epithelialization describes the resurfacing of a skin wound with new epithelium. In response to various stimuli including that of growth factors, cytokines and extracellular matrix (ECM), wound edge epidermal keratinocytes undergo cytoskeleton rearrangements compatible with their motile behavior and develop...
Robyn Nagel et al.
Parasites & vectors, 8, 453-453 (2015-09-17)
Blastocystis species are common enteric human parasites and carriage has been linked to Irritable Bowel Syndrome (IBS), particularly diarrhoea-predominant IBS. The spectrum of immune reactivity to Blastocystis proteins has been reported previously in symptomatic patients. We investigated differences in serum...

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