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444258

Sigma-Aldrich

MMP-14 Substrate I, Fluorogenic

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

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Synonym(s):
MMP-14 Substrate I, Fluorogenic, MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
Empirical Formula (Hill Notation):
C66H82N16O17S
Molecular Weight:
1403.52

Quality Level

assay

≥97% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 5 mg/mL

fluorescence

λex ~328 nm
λem ~400 nm

shipped in

ambient

storage temp.

−20°C

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Sigma-Aldrich

444258

MMP-14 Substrate I, Fluorogenic

Sigma-Aldrich

444256

MMP Substrate III, Fluorogenic

Sigma-Aldrich

444212

MMP-2 Substrate, Fluorogenic

assay

≥97% (HPLC)

assay

≥97% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, protect from light

General description

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (kcat/km = 730,000 M-1s-1 for MMP-14 (MT1-MMP) and 36,000 M-1s-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.
A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (kcat/Km = 7.3 x 105 M-1s-1 for MMP-14 (MT1-MMP) and 3.6 x 104 M-1s-1 for MMP-11 at 25°C, pH 7.5).

Biochem/physiol Actions

Cell permeable: no
Primary Target
MMP-14
Product does not compete with ATP.
Reversible: no
kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

MCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Mucha, A., et al. 1998. J. Biol. Chem.273, 2763.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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