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MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli

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Synonym(s):
ERK1, MAPK3, p44 MAPK

recombinant

expressed in E. coli

Quality Level

assay

≥95% (SDS-PAGE)

form

liquid

specific activity

≥6000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

−70°C

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This Item
218799218785171536
specific activity

≥6000 units/mg protein

specific activity

≥6000 units/mg protein

specific activity

≥150,000 units/mg protein, ≥50,000 units/mL

specific activity

-

form

liquid

form

lyophilized

form

liquid

form

liquid

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in insect cells (S. frugiperda)

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Stephanie Belanger-Jasmin et al.
Journal of neurochemistry, 103(5), 2022-2034 (2007-09-18)
Hairy/Enhancer of split (Hes) 6 is a basic helix-loop-helix protein that interacts with the transcriptional co-repressor, Groucho, and antagonizes the neural functions of the Notch pathway. More specifically, mouse Hes6 regulates cerebral corticogenesis by promoting neurogenesis and suppressing astrocyte differentiation.

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