475904-M

Millipore

MOWIOL® 4-88 Reagent

Synonym(s):
Poly(vinyl alcohol)
Linear Formula:
[-CH2CHOH-]n
CAS Number:
MDL number:

storage conditions

+15C to +30C

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow

storage temp.

10-30°C

InChI

1S/C2H4O/c1-2-3/h2-3H,1H2

InChI key

IMROMDMJAWUWLK-UHFFFAOYSA-N

General description

Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.

Warning

Toxicity: Standard Handling (A)

Preparation Note

Add 2.4 g of MOWIOL® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.

Other Notes

Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Mowiol is a registered trademark of Kuraray Europe GmbH

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.