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475911

Sigma-Aldrich

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

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Synonym(s):
Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
CAS Number:
Enzyme Commission number:

biological source

human

Quality Level

Assay

≥95% (SDS-PAGE)

form

lyophilized

specific activity

150-200 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: soluble (volume is lot-specific and can be found on the product label)

shipped in

wet ice

storage temp.

−20°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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This Item
M6908E8140SAB4701060
assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

specific activity

150-200 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥50 units/mg protein (Bradford)

specific activity

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

General description

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

Physical form

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Preparation Note

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Other Notes

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Shobini Jayaraman et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(8), 158712-158712 (2020-04-15)
Low-density lipoprotein (LDL) binding to arterial proteoglycans initiates LDL retention and modification in the arterial wall, triggering atherosclerosis. The details of this binding, its effectors, and its ramifications are incompletely understood. We combined heparin affinity chromatography with biochemical, spectroscopic and

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