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Sigma-Aldrich

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

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Synonym(s):
MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
Molecular Weight:
414.32
MDL number:

Quality Level

assay

≥98% (silver nitrate titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.5% Sulfated ash

color

yellow

solubility

PBS: 2 mg/mL

cation traces

heavy metals: ≤0.001%

shipped in

ambient

storage temp.

15-25°C

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

General description

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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