480060

Necrosis Inhibitor, IM-54

The Necrosis Inhibitor, IM-54, also referenced under CAS 861891-50-1, selectively blocks oxidative stress-induced necrotic cell death. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Necrosis Inhibitor, IM-54, 2-(1H-Indol-3-yl)-3-pentylamino-maleimide
• Empirical Formula (Hill Notation): C₁₉H₂₃N₃O₂
• CAS Number: 861891-50-1
• Molecular Weight: 325.40
• MDL number: MFCD18382109

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow-orange
• solubility: methanol: 10 mg/mL; DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable mono-indolylmaleimide compound that selectively blocks oxidative stress-induced necrotic cell death (~3 µM IM-54 prevented ~50% cell death in HL60 cells exposed to 100 µM H₂O₂). Does not offer protection against Etoposide- (Cat. No. 341205) induced apoptosis or display antioxidant properties. Does not affect the kinase activities of S6K1 and PKC isozymes even at concentrations as high as 50 µM.

A cell-permeable mono-indolylmaleimide compound that selectively blocks oxidative stress-induced necrotic cell death (~3 µM IM-54 prevented ~50% cell death in HL60 cells exposed to 100 µM H₂O₂). Does not offer protection against Etoposide- (Cat. No. 341205) induced apoptosis or display antioxidant properties. Does not affect the kinase activities of S6K1 and PKC isozymes even at concentrations as high as 50 µM.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Blocks oxidative stress-induced necrotic cell death

Product does not compete with ATP.

Reversible: no

Target IC₅₀: (~3 µM prevented ~50% cell death in HL60 cells exposed to 100 µM H₂O₂).

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Dodo, K., et al. 2005. Bioorg. Med. Chem. Lett.15, 3114.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable