MPEQ-AB is a 10X buffer that can serve as a wash buffer in the Cell Signaling kits when employing an automatic plate washer. It contains lower detergent concentrations compared to 43-041 and should not be utilized as the assay buffer in the kit. MPEQ-AB should specifically be used in the wash steps.
48-611MAG
MILLIPLEX® Akt/mTOR Signaling Panel
Premixed Akt/mTOR Phosphoprotein 11-Plex Cell Signaling Panel
Synonym(s):
Akt/mTOR Signaling immunoassay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Assay, 11-plex assay, Akt mTOR assay, Akt phosphorylation, PI3K/Akt/mTOR pathway, cell signaling multiplex, kinase signaling panel, mTOR signaling, phospho-Akt, phospho-mTOR
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About This Item
NACRES:
NA.47
UNSPSC Code:
12161503
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Quality Level
species reactivity
human
technique(s)
multiplexing: suitable
input
cell lysate
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
detection method
fluorometric (Luminex® xMAP®technology)
shipped in
wet ice
storage temp.
2-8°C
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This Item | 48-612MAG | 48-680MAG | 48-614MAG |
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex® xMAP®) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
This panel includes key phosphoproteins from the PI3K/Akt/mTOR pathway such as Akt (pS473, pT308), mTOR (pS2448), p70 S6K, RPS6, 4E-BP1, and GSK-3α/β. These analytes regulate cell survival, growth, protein synthesis, and metabolism through coordinated phosphorylation events.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 11 analytes in cell lysate samples.
Analytes Included: Akt (pS473), Akt (pT308), GSK-3α/β (pS21/S9), mTOR (pS2448), p70 S6K (pT412), p70 S6K (pT389), p70 S6K (Total), RPS6 (pS235/S236), RPS6 (pS240/S244), RPS6 (Total), 4E-BP1 (pT37/T46)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Analytes Included: Akt (pS473), Akt (pT308), GSK-3α/β (pS21/S9), mTOR (pS2448), p70 S6K (pT412), p70 S6K (pT389), p70 S6K (Total), RPS6 (pS235/S236), RPS6 (pS240/S244), RPS6 (Total), 4E-BP1 (pT37/T46)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
- Broad coverage of the PI3K/Akt/mTOR signaling pathway
- Detects multiple phosphorylation sites and total proteins
- Enables pathway mapping and mechanistic studies
- High multiplexing reduces sample and reagent consumption
- Optimized for reproducibility and sensitivity
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
signalword
Danger
Storage Class
10 - Combustible liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
Certificates of Analysis (COA)
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What is the difference in formulation between the MPEQ-AB Assay Buffer and 43-041 Cell Signaling Assay Buffer 2? Can they be used interchangeably?
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