The 2-plex Cell Signaling kits can be combined except for the Akt panels. The Akt Panels cannot be combined with another Akt panel but they can be combined with any other 2-plex kit.
48-619MAG
MILLIPLEX® Total/Phospho Erk - Cell Signaling Multiplex Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
Pricing and availability is not currently available.
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species reactivity
human, mouse, rat
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-618MAG) and Total/Phospho ERK (48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)Analytes available:Erk (Total)Erk (Thr185/Tyr187)
Packaging
96-well plate
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Related Content
Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.
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Is it possible to combine the Total/Phospho 2-Plex Cell Signaling Kits including the following components: 48-618MAG - Akt, 48-631MAG - Akt1, 48-632MAG - Akt2, 48-633MAG - Akt3, 48-628MAG - CREB, 48-619MAG - Erk/MAPK1/2, 48-626MAG - IRS1, 48-622MAG - JNK, 48-625MAG - mTOR, 48-624MAG - p38, 48-623MAG - STAT3?
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