MilliporeSigma
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48-619MAG

Millipore

MILLIPLEX® Total/Phospho Erk - Cell Signaling Multiplex Assay

All Photos(1)

species reactivity

human, mouse, rat

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

storage temp.

2-8°C

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Millipore

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48-619MAG

MILLIPLEX® Total/Phospho Erk - Cell Signaling Multiplex Assay

Millipore

Millipore

48-618MAG

MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay

Millipore

Millipore

48-628MAG

MILLIPLEX® 2-Plex Phospho/Total CREB - Cell Signaling Multiplex Assay

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Millipore

48-660MAG

MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

species reactivity

human, mouse, rat

species reactivity

human, mouse, rat

species reactivity

human, rat, mouse

species reactivity

human

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex xMAP)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-618MAG) and Total/Phospho ERK (48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)Analytes available:Erk (Total)Erk (Thr185/Tyr187)

Packaging

96-well plate

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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