MILLIPLEX® Total/Phospho Erk - Cell Signaling Multiplex Assay

Pricing and availability is not currently available.

species reactivity

mouse, rat, human

Quality Level

mfr. no.



multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

storage temp.


General description

The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-618MAG) and Total/Phospho ERK (48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.


Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.


Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)Analytes available:Erk (Total)Erk (Thr185/Tyr187)


96-well plate

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificate of Analysis
Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.