48-622MAG
MILLIPLEX® 2-Plex Phospho/Total JNK Magnetic Bead Kit - Cell Signaling Multiplex Assay
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species reactivity
human, rat, mouse
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
Related Categories
General description
The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-622MAG) has been developed for the simultaneous detection of phosphorylated JNK (Thr183/Tyr185) and total JNK in a single well using the Luminex system.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: Erk (Total), Erk (Thr185/Tyr187).This is an overnight (4°C) incubation assay.This kit must be run using the provided Assay Buffer.This assay requires 25 μL diluted filtered cell lysate per well.The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:JNK (Total);JNK (Thr183/Tyr185)
Packaging
96-well plate
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
wgk_germany
WGK 3
Certificates of Analysis (COA)
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MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
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