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48-660MAG

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MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

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eCl@ss:
32161000

species reactivity

human

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

assay range

inter-assay cv: 4-13%
intra-assay cv: 3-10%

detection method

fluorometric (Luminex xMAP)

storage temp.

2-8°C

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MILLIPLEX® 2-Plex Phospho/Total CREB - Cell Signaling Multiplex Assay

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

assay range

inter-assay cv: 4-13%
intra-assay cv: 3-10%

assay range

-

assay range

-

assay range

-

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex® xMAP®)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • An overnight (4°C) incubation is recommended for best results.
  • This assay requires 25 μL diluted cell lysate.
  • This kit must be run using Assay Buffer 2 (provided).
  • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Research Category
Signaling
Used to detect/quantify:ATF2 (Thr71)|Erk (Thr185/Tyr187)|HSP27 (Ser78)|JNK (Thr183/Tyr185)|c-Jun (Ser73)|MEK1 (Ser222)|MSK1 (Ser212)|p38 (Thr180/Tyr182)|p53 (Ser15)|STAT1 (Tyr701)

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Signal Word

Danger

Hazard Statements

H302 - H315 - H318 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Cheng-Wei Wu et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 246-247, 110461-110461 (2020-06-05)
In arid conditions, the African Clawed frog Xenopus laevis enters a state of estivation dormancy as an adaptive survival strategy. Under estivation, X. laevis experience severe dehydration stress as 25-35% of total body water is lost. Dehydration in X. laevis

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