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48-681MAG

Millipore

MILLIPLEX® Multi-Pathway Total Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay

Synonym(s):
Luminex Cell Signaling Assay, Signal Transduction Panel, Millipore Cell Signaling Assay

Quality Level

species reactivity

mouse, human, rat

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

storage temp.

2-8°C

General description

The MILLIPLEX® ColA MAP 9-plex Multi-Pathway Total Magnetic Bead kit is used to detect total protein levels for ERK/MAP kinase 1/2, Akt, STAT3, JNK, p70 S6 kinase, NFκB, STAT5A/B, CREB, and p38 in cell lysates using the Luminex® xMAP technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.Analytes available:Erk/MAPK 1/2 (Total);Akt (Total);STAT3 (Total);JNK (Total);p70 S6 Kinase (Total);NFκB (Total);STAT5A/B (Total);CREB (Total);p38 (Total)

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificate of Quality

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