The Milliplex® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
- An overnight (4°C) incubation is recommended for best results.
- This assay requires 25 μL diluted cell lysate.
- This kit must be run using Assay Buffer 2 (provided).
- 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
Used to detect/quantify:CD3ε (pan Tyr)|CREB (S133)|ERK (Thr185/Tyr187)|LAT (pan Tyr)|Lck (pan Tyr)|Syk (pan Tyr)|ZAP-70 (pan Tyr)
Storage and Stability
Recommended storage for kit components is 2 - 8°C
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MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
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