5010-OP

Guanidine Hydrochloride

OmniPur^® Grade, >= 99.5%, Denaturant used for purification of nucleic acids and polypeptides from cell lysates

• Synonym(s): Guanidine hydrochloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Linear Formula: NH₂C(=NH)NH₂ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• Beilstein/REAXYS Number: 3591990
• MDL number: MFCD00013026
• UNSPSC Code: 12352107
• NACRES: NA.28

Properties

• Quality Level: 100
• form: crystalline powder (granular)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• mp: 180-185 °C (lit.)
• density: 1.3 g/cm³ (lit.)
• foreign activity: DNAse, none detected; Phosphatase, none detected; Protease, none detected; RNAse, none detected
• shipped in: ambient
• storage temp.: 10-30°C
• SMILES string: Cl[H].NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N

Description

General description

Denaturant used for purification of nucleic acids and polypeptides from cell lysates. Inhibits RNase and protease activity. Molecular Biology grade.br
A₂₆₀ (0.1 M, water): ≤0.05 AU
A₂₈₀ (0.1 M, water): ≤0.01 AU
Purity by Assay: ≥99.5%
DNase: None detected
Heavy metals (as Pb): ≤0.001%
Phosphatase: None detected
Protease: None detected
RNase: None detected





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H332,H315,H319
• pcodes: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable