MilliporeSigma
All Photos(1)

5.06416

Sigma-Aldrich

Beclin-1 Activator II, retro-inverso TAT-Beclin-1 - Calbiochem

Beclin-1 Activator II, retro-inverso TAT-Beclin-1 is a cell-permeable, all-D-amino acid autophagy-inducing peptide based on the retro-inverso sequence of Tat-Beclin1 (Cat. No. 506048).

Synonym(s):
Beclin-1 Activator II, retro-inverso TAT-Beclin-1 - Calbiochem, (D)-H₂N-RRRQRRKKRGY-GG-TGFEGDHWIEFTANFVNT-CO₂H, Atg6 Activator II, Beclin-1-GAPR-1 Interaction Blocker II, Vps30 Activator II
Empirical Formula (Hill Notation):
C164H251N57O45
Molecular Weight:
3741.10

assay

≥95% (HPLC)

Quality Level

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

white

solubility

DMSO: 50 mg/mL
water: 50 mg/mL

storage temp.

2-8°C

General description

A cell-permeable, all-D-amino acid autophagy-inducing peptide based on the retro-inverso sequence of Tat-Beclin1 (Cat. No. 506048) that is expected to be more resistant to proteolytic degradation than Tat-Beclin1 and is shown to be more potent than Tat-Beclin1 in inducing autophagy in muscle, heart, and pancreas tissues in 6-wk-old GFP-LC3 transgenic mice (1.94-, 1.48-, and 1.16-fold of Tat-Beclin1 GFP-LC3 induction potency in respective tissue in 6 h; 20 mg/kg i.p.) in vivo. Only the retro-inverso peptide, but not Tat-Beclin1, is effective in reducing autophagy substrate p62 level in the brain tissue of 5-d-old neontal GFP-LC3 mice (20 mg/kg i.p.). The retro-inverso peptide treatment is also reported to greatly reduce brain viral titre in neonatal mice subjected to WNV (West Nile Virus) CNS (central nervous system) infection (>1000-fold reduction 6 d post infection; 20 mg/kg/d i.p. beginning 1 d post infection), while being ineffective against WNV CNS infection in mice with monoallelic deletion of the Beclin1 gene Becn1.

Packaging

5 mg in Glass bottle
Packaged under inert gas

Biochem/physiol Actions

Cell permeable: yes
Primary Target
GAPR-1/GLIPR2
Reversible: yes

Warning

Toxicity: Standard Handling (A)

Sequence

(D)-H-arg-arg-arg-gln-arg-arg-lys-lys-arg-Gly-tyr~Gly-Gly~thr-Gly-phe-glu-Gly-asp-his-trp-ile-glu-phe-thr-ala-asn-phe-val-asn-thr-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Shoji-Kawata, S., et al. 2013. Nature.494, 201.

Legal Information

Sold under license of US Patent 8,722,628.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service