507861
PANSORBIN® Cells, Standardized
Synonym(s):
Cell standard
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About This Item
NACRES:
NA.42
UNSPSC Code:
41116133
Manufacturer/tradename:
Calbiochem®
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form
liquid
contains
0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
capacity
≥2 mg/mL binding capacity
shipped in
ambient
Quality Level
1 of 4
This Item | CLLS1048 | S2014 | CLLS1107 |
|---|---|---|---|
| storage temp. 2-8°C | storage temp. −196°C | storage temp. 2-8°C | storage temp. −196°C |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| form liquid | form - | form buffered aqueous suspension | form - |
| contains 0.1% sodium azide as preservative | contains - | contains - | contains - |
General description
Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high-affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3).
PANSORBIN Cells are heat-killed, formalin fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lymphocytes and for immunoprecipitation.
Application
Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
Physical form
Supplied as a ≥10% (w/v) Staphylococcus aureus cell suspension in PBS, 0.1% sodium azide, pH 7.2.
Other Notes
Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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