A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.
Packaged under inert gas
Toxicity: Toxic (F)
Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany