5.08605

Sigma-Aldrich

Sodium Orthovanadate, Activated - Calbiochem

A depolymerized vanadate prepared from Sodium Orthovanadate with enhanced ability to competitively inhibit endogenous phosphatases.

Synonym(s):
Sodium Orthovanadate, Activated - Calbiochem, Orthovanadate, Activated, Vanadate, Activated
Empirical Formula (Hill Notation):
Na3O4V
Molecular Weight:
183.91

storage conditions

= -70C

form

liquid

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

color

colorless

storage temp.

−70°C

General description

A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.

Biochem/physiol Actions

Primary Target
Phosphatases
Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Toxic (F)

Physical form

Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Other Notes

Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

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