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5.08741

Millipore

DAPI Stain

All Photos(1)
Synonym(s):
DAPI Stain, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
28718-90-3
Molecular Weight:
350.25
MDL number:
MFCD00012681

Assay

≥97% (HPLC)

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

fluorescence

λex ~359 nm
λem ~461 nm

storage temp.

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Inchi Key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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This Item
D954226829810236276001
DAPI Stain

Millipore

5.08741

DAPI Stain

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

DAPI, Dihydrochloride Cell-permeable DNA-binding dye.

Sigma-Aldrich

268298

DAPI, Dihydrochloride

DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

Roche

10236276001

DAPI

form

liquid

form

powder

form

solid

form

powder

fluorescence

λex ~359 nm, λem ~461 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex ~359 nm, λem ~461 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Roche

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
DNA
Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical form

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.

Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.

Russels, W.C., et al. 1975. Nature253, 461.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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