521270

PDK1, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(s): 3-Phosphoinositide-Dependent Protein Kinase-1
• Enzyme Commission number: 2.7.10
• UNSPSC Code: 12352202

Properties

• assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥75 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full length, recombinant, human PDK1 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKC_ζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Scott, M.T., et al. 2002. EMBO J.21, 6771.
Biondi, R.M., et al. 2000. EMBO J.19, 979.
Alessi, D.R., et al. 1997. Curr. Biol.7, 776.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2