MilliporeSigma
All Photos(1)

Documents

52332

Sigma-Aldrich

Phenylmethylsulfonyl Fluoride

Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.

All Photos(1)
Synonym(s):
Phenylmethylsulfonyl Fluoride, Benzylsulfonyl Fluoride, PMSF
Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
329-98-6
Molecular Weight:
174.19
MDL number:
MFCD00007424
NACRES:
NA.77

Quality Level

200

Assay

≥99% (GC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white to off-white

solubility

ethanol: soluble
isopropanol: soluble
methanol: soluble

shipped in

ambient

storage temp.

10-30°C

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P76267883093482
Phenylmethylsulfonyl Fluoride Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.

Sigma-Aldrich

52332

Phenylmethylsulfonyl Fluoride

Phenylmethanesulfonyl fluoride ≥98.5% (GC)

Sigma-Aldrich

P7626

Phenylmethanesulfonyl fluoride

Phenylmethanesulfonyl fluoride ≥99.0% (T)

Sigma-Aldrich

78830

Phenylmethanesulfonyl fluoride

Phenylmethanesulfonyl fluoride solution ~0.1 M in ethanol (T)

Sigma-Aldrich

93482

Phenylmethanesulfonyl fluoride solution

form

crystalline solid

form

powder

form

powder or crystals

form

liquid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

storage condition

-

color

white to off-white

color

-

color

-

color

-

solubility

ethanol: soluble, isopropanol: soluble, methanol: soluble

solubility

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable

solubility

anhydrous isopropanol: soluble 35 mg/mL, clear to faintly hazy, colorless to faintly yellow (Heating required)

solubility

-

General description

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. Effective at a concentration of 50 µM.
Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).

Biochem/physiol Actions

Cell permeable: no
Primary Target
serine proteases
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Corrosive (L)

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.
t½ = 1 hour at pH 7.5. Effective concentration: 50 μM.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Seitz, R., et al. 1993. Int. J. Cancer53, 514.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionSkull and crossbones
GHS05,GHS06

Signal Word

Danger

Hazard Statements

H301 - H314

Hazard Classifications

Acute Tox. 3 Oral - Skin Corr. 1B

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 10

1 of 10

Leupeptin microbial, ≥90% (HPLC)

Sigma-Aldrich

L2884

Leupeptin

Benzamidine hydrochloride hydrate ≥99%

Sigma-Aldrich

B6506

Benzamidine hydrochloride hydrate

Pepstatin A microbial, ≥90% (HPLC)

Sigma-Aldrich

P5318

Pepstatin A

Pepstatin from Streptomyces species

Roche

PEPS-RO

Pepstatin

Sodium fluoride BioXtra, ≥99%

Sigma-Aldrich

S7920

Sodium fluoride

Hydroxyproline Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK008

Hydroxyproline Assay Kit

Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Sigma-Aldrich

L6876

Lysozyme from chicken egg white

Pefabloc® SC powder, solubility: 100 mg/mL in aqueous buffer, suitable for blocking, suitable for protein purification

Roche

PEFBSC-RO

Pefabloc® SC

Sigma-Aldrich

Sigma-Aldrich

P2714

Protease Inhibitor Cocktail powder

Sigma-Aldrich

Sigma-Aldrich

P8340

Protease Inhibitor Cocktail

Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2.
Takashima, et al.
Journal of biological methods, 10, e99010002-e99010002 (2023)
Ming Chang et al.
Bio-protocol, 7(8), e2227-e2227 (2017-04-20)
Arabidopsis pollen is an excellent system for answering important biological questions about the establishment and maintenance of cellular polarity and polar cell growth, because these processes are amenable to the genetic and genomic approaches that are readily available in Arabidopsis.
Siqi Ming et al.
Frontiers in immunology, 12, 736269-736269 (2021-10-01)
Gastric CD4+T cells contribute to Helicobacter pylori (H. pylori)-induced gastritis by amplifying mucosal inflammation and exacerbating mucosal injuries. However, the pathogenic CD4+ T cell subset involved in gastritis and the potential regulators are still unclear. Here we identified an IL-21-producing
Fabrizio Clarelli et al.
PLoS computational biology, 16(8), e1008106-e1008106 (2020-08-17)
Antibiotic resistance is rising and we urgently need to gain a better quantitative understanding of how antibiotics act, which in turn would also speed up the development of new antibiotics. Here, we describe a computational model (COMBAT-COmputational Model of Bacterial
Chongyuan Luo et al.
Methods in molecular biology (Clifton, N.J.), 1112, 177-193 (2014-01-31)
Genome-wide quantitative profiling of chromatin modifications is a critical experimental approach to study epigenetic and transcriptional control mechanisms. Since first being reported in 2007, chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) has soon became a popular method-of-choice for profiling chromatin
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service