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524739

Sigma-Aldrich

Phosphodiesterase 10A2, His•Tag®, Human, Recombinant, S. frugiperda

Synonym(s):

PDE10A2

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About This Item

EC Number:
3.1.4.17 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in baculovirus infected Sf21 cells

Quality Level

100

form

liquid

specific activity

≥0.25 U/μL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

Storage temp.

−70°C

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specific activity

≥0.25 U/μL

specific activity

≥0.5 U/μL

specific activity

≥0.25 U/μL

specific activity

≥0.25 U/μL

form

liquid

form

liquid

form

liquid

form

liquid

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in insect cells (S. frugiperda)

recombinant

expressed in baculovirus infected Spodoptera frugiperda cells

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His•Tag7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.

Physical form

In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.

Other Notes

Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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