525200-M

Phospholipase D, Streptomyces chromofuscus

Name: Phospholipase D, <i>Streptomyces chromofuscus</i>
Brand: MM
Price: 364 USD

Synonym(s):

Phospholipase D, Streptomyces chromofuscus, PLD

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525200-250U

$364.00

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About This Item

CAS Number:

9001-87-0

EC Number:

3.1.4.4 (BRENDA, IUBMB)

MDL number:

MFCD00131897

UNSPSC Code:

12352202

NACRES:

NA.54

Key Documents

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form

lyophilized solid

Quality Level

100

specific activity

≥50 units/mg dry wt.

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated

color

brownish

solubility

10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON^™ X-100: 1 mg/mL

foreign activity

Aatalase, glucose oxidase, none detected

storage temp.

−20°C

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This Item	P0065	228250	525186
Sigma-Aldrich 525200-M Phospholipase D, Streptomyces chromofuscus	Sigma-Aldrich P0065 Phospholipase D from Streptomyces chromofuscus	Sigma-Aldrich 228250 Cholesterol Oxidase, Streptomyces sp.	Sigma-Aldrich 525186 Phospholipase C, Bacillus cereus
specific activity ≥50 units/mg dry wt.	specific activity ≥50,000 units/mL	specific activity ≥3 units/mg solid, ≥40 U/mg	specific activity ≥50 units/mg dry wt.
form lyophilized solid	form buffered aqueous glycerol solution	form lyophilized solid	form solid
solubility 10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON^™ X-100: 1 mg/mL	solubility -	solubility water: 20 mg/mL	solubility aqueous buffer: soluble, water: soluble
storage temp. −20°C	storage temp. −70°C	storage temp. −20°C	storage temp. −20°C
storage condition OK to freeze, desiccated	storage condition -	storage condition OK to freeze, desiccated, protect from light	storage condition OK to freeze, desiccated (hygroscopic)
foreign activity Aatalase, glucose oxidase, none detected	foreign activity -	foreign activity Catalase ≤1%, glucose oxidase and uricase ≤0.01%	foreign activity -

General description

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.

Other Notes

Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Harmful (C)

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 + P312 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Certificates of Analysis (COA)

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