Recognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Phosphotyrosine.
Immunogen
purified phosphotyrosine conjugated to KLH
Application
ELISA (1:5000-1:10,000)
Immunoblotting (1:1000-1:2000)
Immunoprecipitation (2-5 µg)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Other Notes
Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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