528180

Plasminogen, Glu-Type, Human Plasma

• Synonym(s): Glu-type plasminogen, Plasminogen
• NACRES: NA.25
• UNSPSC Code: 12352202

Properties

• description: Merck USA index - 14, 7521
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• foreign activity: Plasmin, none detected
• shipped in: wet ice
• storage temp.: −70°C
• Quality Level: 100

Description

Analysis Note

Single band by SDS-PAGE

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Toxicity: Irritant (B)

General description

Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein zymogen, which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 µM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls, and 5 regions of internal sequence homology, known as kringles, between Lys⁷⁷ and Arg⁵⁶⁰. These kringle regions are homologous to the kringle domains in t-PA, u-PA, and prothrombin. Native Glu-plasminogen is readily converted to Lys⁷⁷-plasminogen by plasmin hydrolysis of the Lys⁷⁶-Lys⁷⁷ peptide bond. The plasminogen is converted to plasmin by cleavage at Arg⁵⁴⁰-Val⁵⁶¹ by urokinase, streptokinase, or tissue plasminogen activator.

Other Notes

Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243.
Barlow, G.H., et al. 1984. Biochemistry23, 2384.
Wohl, R.C., et al. 1982. Thromb. Res.27, 523.
Castellino, F.J., et al. 1981. Methods Enzymol.80, 365.
Robbins, K.C. 1976. Methods Enzymol.45, 257.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable